RNA Sequencing Assay:Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Expressing:Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Western Blot:Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Concentration Assay:Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.
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