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anti e2f1  (OriGene)


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    Structured Review

    OriGene anti e2f1
    Anti E2f1, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+e2f1/E2F1+(acetyl+K120%2FK125)+Rabbit+Polyclonal+Antibody/pmc10010392-59-31-35
    Average 91 stars, based on 1 article reviews
    anti e2f1 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Expressing:

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Western Blot:

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Concentration Assay:

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ]. .. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore). .. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.

    Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
    Article Snippet: Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].Western blotting Whole cell protein isolation from cultured cells and harvested tumors, hydrochloric acid extraction of histones, western blotting and signal detection were as described [ 19 ].. Antibodies used were rabbit polyclonal anti-cyclin E (Abcam, Cambridge, MA), rabbit polyclonal anti-E2F1 (DBA Acris Antibodies, Inc, Rockville, MD), rabbit polyclonal anti-RAD51 (Millipore), mouse monoclonal anti-BRCA1 (Millipore), rabbit polyclonal anti-PARP (Cell Signaling Technology), mouse monoclonal anti-PCNA (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit polyclonal anti-cleaved caspase-3 (Cell Signaling Technology), and mouse monoclonal anti-pH2AX (Ser139) (Millipore).. Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.Loading controls were mouse monoclonal anti-histone H3 (Millipore) and mouse monoclonal β-actin (Sigma) for histones and total proteins, respectively.



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    Cell Signaling Technology Inc rabbit polyclonal e2f1
    Fig. 7. <t>E2F1</t> is stabilized in response to MPO. (A) HCT116 p53WT and (B) p53−/−cells were treated with MPO (20 μM) for 24–96 h and lysates from the nuclear and cytosolic fractions were subjected to Western blotting analysis using anti-E2F1 and anti-β actin antibodies. (C) HCT p53WT cells were treated with MPO (20 μM) for 24 h in the presence or absence of cycloheximide (CHX) (2.5–5 μg/ml) or actinomycin D (Act. D) (0.5 μg/ml) and cell lysates were subjected to Western blotting analysis and probed with anti-E2F1 or anti-β actin antibodies.
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    Image Search Results


    Journal: STAR Protocols

    Article Title: Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays

    doi: 10.1016/j.xpro.2023.102109

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-E2F1 , Cell Signaling Technology , CAT#3742S.

    Techniques: Western Blot, Virus, Bacteria, Recombinant, Protease Inhibitor, Magnetic Beads, Plasmid Preparation, Control, Software, Blocking Assay, Inverted Microscopy, Transferring, Spectrophotometry, Hood

    Fig. 7. E2F1 is stabilized in response to MPO. (A) HCT116 p53WT and (B) p53−/−cells were treated with MPO (20 μM) for 24–96 h and lysates from the nuclear and cytosolic fractions were subjected to Western blotting analysis using anti-E2F1 and anti-β actin antibodies. (C) HCT p53WT cells were treated with MPO (20 μM) for 24 h in the presence or absence of cycloheximide (CHX) (2.5–5 μg/ml) or actinomycin D (Act. D) (0.5 μg/ml) and cell lysates were subjected to Western blotting analysis and probed with anti-E2F1 or anti-β actin antibodies.

    Journal: Cancer letters

    Article Title: Identification of a novel catalytic inhibitor of topoisomerase II alpha that engages distinct mechanisms in p53 wt or p53 -/- cells to trigger G2/M arrest and senescence.

    doi: 10.1016/j.canlet.2021.11.025

    Figure Lengend Snippet: Fig. 7. E2F1 is stabilized in response to MPO. (A) HCT116 p53WT and (B) p53−/−cells were treated with MPO (20 μM) for 24–96 h and lysates from the nuclear and cytosolic fractions were subjected to Western blotting analysis using anti-E2F1 and anti-β actin antibodies. (C) HCT p53WT cells were treated with MPO (20 μM) for 24 h in the presence or absence of cycloheximide (CHX) (2.5–5 μg/ml) or actinomycin D (Act. D) (0.5 μg/ml) and cell lysates were subjected to Western blotting analysis and probed with anti-E2F1 or anti-β actin antibodies.

    Article Snippet: The following antibodies were used in the study: Mouse monoclonal β-actin (Sigma Aldrich Co., St. Louis, MO), mouse monoclonal p53, p21, topo IIα, (BD Pharmingen, San Diego, CA, USA), mouse monoclonal phospho-p53(ser15), phospho-ATM(ser1981) (Cell Signaling Technology Inc., Danvers, MA), mouse monoclonal ATM, Chk2, Chk1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA), mouse monoclonal phosphohistone H2AX(ser139), clone JBW301 (Upstate Biotechnology Inc., Lake Placid, NY), rabbit monoclonal phospho-Chk2(thr68), phospho-Chk1 (ser345) (Cell Signaling Technology Inc., Danvers, MA), rabbit polyclonal E2F1 (Cell Signaling Technology Inc., Danvers, MA), rabbit polyclonal topo IIα (TopoGEN, Inc., Columbus, OH), rabbit polyclonal ATR (Calbiochem, San Diego, CA), goat anti-mouse IgG HRP conjugated and goat anti-rabbit IgG HRP conjugated secondary antibodies (Pierce Chemical Co., Rockford, IL).

    Techniques: Western Blot